Kasp snp genotyping manual

KASP version SNP Genotyping Manual v 5 Sample Arraying DNA samples may be arrayed in any microtitre PCR plate though typica or ‐well plates are used. The recommended amounts of DNA to use are: 5μl of DNA for 96‐well plates and μl of DNA (1‐40ng/µL) for. This document is intended as a guide to running KASPTM genotyping reactions on the. The KASP genotyping assay utilizes a unique form of competitive allele-specific PCR combined with a novel, homogeneous, fluorescence-based reporting system for the identification and measurement of genetic variation occurring at the nucleotide level to detect single nucleotide polymorphisms (SNPs) o Cited by: KASP genotyping technology is based on competitive allele-specific PCR and enables bi-allelic scoring of single nucleotide polymorphisms (SNPs) and insertions and deletions (Indels) at specific loci. KASP is easy to use – simply add your SNP-specific KASP Assay Mix and the universal KASP-TF Master Mix to your DNA samples, run a thermal cycling program, and measure the end-point fluorescence.

This document is intended as a guide to running KASP® genotyping reactions on the Applied Biosystems QuantStudio 6 7 instruments. KASP chemistry for allelic discrimination performs well on QuantStudio instruments and this step-by-step protocol will enable you to successfully set-up, run and read plates on the QuantStudio 6 7. 2. The KASP genotyping reaction comprises three components: Sample DNA with the sequence of interest; KASP Assay mix (containing the target specific primers) KASP-TF Master Mix. KASP-TF Master Mix and KASP Assay mix are used in-house for our genotyping service projects but can also be purchased to run KASP genotyping reactions in your own laboratory. KASP™ genotyping assays are based on competitive allele-specific PCR and enable bi-allelic scoring of single nucleotide polymorphisms (SNPs) and insertions and deletions (Indels) at specific loci. KASP-TF Master Mixes are formulated without Triton™ X The SNP-specific KASP Assay mix and the universal KASP-TF Master Mix are added to DNA samples, a thermal cycling reaction is then performed, followed by an end-point fluorescent read.

A set of SNPs were analyzed by Kompetitive Allele Specific PCR (KASP) assay in order to discriminate the Rubus genotypes. Results. A total of 50,, (%). KASP genotyping manual. Manual. For Research Use Only. single nucleotide polymorphisms (SNPs) and insertions/deletions (indels) at specific loci. KASP. Genotyping assay designs are available by using our free assay design service at www.doorway.ru KASP™, IdentiSNP and Amplifluor® assays can.